Adenoviral Vectors for Gene Therapy, Second Edition by David T. Curiel
By David T. Curiel
Adenoviral Vectors for Gene treatment, moment Edition offers particular, finished insurance of the gene supply cars which are in response to the adenovirus that's rising as a huge software in gene remedy. those intriguing new healing brokers have nice strength for the therapy of sickness, making gene remedy a fast-growing box for learn.
This publication provides subject matters starting from the elemental biology of adenoviruses, throughout the building and purification of adenoviral vectors, state of the art vectorology, and using adenoviral vectors in preclinical animal types, with ultimate attention of the regulatory concerns surrounding human medical gene remedy trials. This wide scope of knowledge presents a fantastic assessment of the sector, permitting the reader to achieve an entire figuring out of the improvement and use of adenoviral vectors.
- Provides entire insurance of the fundamental biology of adenoviruses, in addition to their development, propagation, and purification of adenoviral vectors
- Introduces universal concepts for the improvement of adenoviral vectors, in addition to state-of-the-art tools for his or her improvement
- Demonstrates noninvasive imaging of adenovirus-mediated gene transfer
- Discusses application of adenoviral vectors in animal disorder models
- Considers Federal Drug management rules for human scientific trials
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Extra info for Adenoviral Vectors for Gene Therapy, Second Edition
Adenoviruses are icosahedral particles of pseudo T = 25 triangulation, in which each facet has 12 trimers of the major coat protein hexon. The hexons contain exposed Adenoviral Vectors for Gene Therapy. 00002-4 Copyright © 2016 Elsevier Inc. All rights reserved. Figure 1 Schematic depiction of HAdV-C2/5 entry into epithelial cells. 55,56 Interleukin-8 binds to the CXCR1/2 receptor and triggers translocation of CAR and integrin receptors to the apical surface of epithelial cells. 40 Secreted lysosomal acid sphingomyelinase (ASMase) generates ceramides.
1 Clathrin-Mediated Endocytosis Clathrin-mediated endocytosis (CME) is initiated at specialized membrane domains rich in phosphatidyl-inositol-4,5 bisphosphate (PI4,5P2). 174,175 Human AdV-C2 and HAdV-C5 gain access into epithelial cells by clathrin- and dynamin-mediated endocytosis. 135,136,182 Many β-integrins harbor an asparagine-proline-any amino acid-tyrosine (NPXY) or NPXY-like motive in their cytoplasmic domain, which binds to cargo selectors such as NUMB and Dab2. NUMB directly interacts with the adapter AP-2 and clathrin.
J Virol 2006;80:11881–6. Boudin ML, D’Halluin JC, Cousin C, Boulanger P. Human adenovirus type 2 protein IIIa. II. Maturation and encapsidation. Virology 1980;101:144–56. Chroboczek J, Viard F, D’Halluin JC. Human adenovirus 2 temperature-sensitive mutant 112 contains three mutations in the protein IIIa gene. Gene 1986;49:157–60. San Martin C, Glasgow JN, Borovjagin A, Beatty MS, Kashentseva EA, Curiel DT, et al. Localization of the N-terminus of minor coat protein IIIa in the adenovirus capsid.